Reaction of Ribosome-bound Peptidyl Transfer Ribonucleic Acid with Aminoacyl Transfer Ribonucleic Acid or Puromycin
نویسندگان
چکیده
منابع مشابه
Reaction of ribosome-bound peptidyl transfer ribonucleic acid with aminoacyl transfer ribonucleic acid or puromycin.
Polylysyl transfer ribonucleic acid, free of lysyl-tRNA, has been isolated and its characteristics in an Escherichiu cofi polypeptide-synthesizing system in vitro have been studied. Ribosomes obtained from frozen E. coli cells were washed in 0.5 M NH&l and assayed at MgCla concentrations of at least 0.01 M. Binding of this peptidyl-tRNA to ribosomes occurs in the presence of templates containin...
متن کاملPreparation of Polyribosome Aminoacyl-Transfer Ribonucleic Acid
A procedure for preparing polyribosome aminoacyl-tRNA free from contamination by supernatant aminoacyl-tRNA and free amino acids is described. Important features of the procedure are the use of acidic buffers to help protect the amino acid-tRNA linkage and the inclusion ofsodium dodecyl sulphate, to inhibit ribonuclease activity. The specific radioactivity of polyribosome aminoacyl-tRNA is high...
متن کاملAffinity chromatography of aminoacyl-transfer ribonucleic acid synthetases. Cognate transfer ribonucleic acid as a ligand.
The use of tRNA affinity columns for the purification of aminoacyl-tRNA synthetases was investigated. A purification method for valyl-tRNA synthetase from Bacillus stearothermophilus is described that uses two affinity columns, one containing the pure cognate tRNA, and the other containing all tRNA species except the cognate tRNA. A method for the rapid preparation of the two columns was develo...
متن کاملThe Function of Pseudouridylic Acid in Transfer Ribonucleic Acid II. INHIBITION OF AMINO ACYL TRANSFER RIBONUCLEIC ACID-RIBOSOME COMPLEX FORMATION
Inhibition of the nonenzymatic binding of phenylalanyl transfer RNA to polyuridylic acid-coded ribosomes at 20 mu magnesium was used as an assay to determine whether the tetranucleotide ribothymidylyl-pseudouridylyl-cytidylyl-guanosine 3’-phosphate possessed any specific ability to bind to ribosomes. Inhibition of binding at the peptidyl site was studied by adding tetracycline to block aminoacy...
متن کاملStudies on transfer ribonucleic acid-ribosome complexes. XXI. Effect of antibiotics on peptidyl-puromycin synthesis by mammalian polyribosomes.
The effect of antibiotics and other agents on-peptide bond synthesis by mammalian polyribosomes was determined by studying peptidyl-puromycin synthesis. Sparsomycin, anisomycin, cycloheximide, gougerotin, blasticidin S, and cryptopleurine inhibited the reaction. Sparsomycin and anisomytin inhibited the peptidyl-puromycin synthesis competitively with respect to puromycin. Arabinose analogs of pu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99394-4